Okazaki fragments

Sorry I've been remiss in posting for a while. I've been busy with my new job, teaching biology. This post isn't about fungi or Alabama, but about what I perceive to be an injustice of sorts. The textbook I'm using makes a big deal about highlighting scientists and their famous experiments; Mendel, Darwin, Watson and Crick (and Rosalind Franklin, of course), Hershey and Chase, Meselson and Stahl, but they just mentioned Okazaki fragments, and glossed over the fact that they were named for the scientist (sic) who discovered them. Okazaki fragments are the short, punctuated stretches of DNA that are produced on the lagging strand when the molecule is being copied.

My curiosity piqued by this oversight, I decided to find out who Okazaki is or was. As it turns out, Okazaki is and was. The eponymous fragments were discovered by a husband and wife team, Reiji and Tsuneko Okazaki, in 1968. Reiji died from leukemia in 1975. He was a native of Hiroshima, and survived the immediate effects of the bombing that ended the Second World War. Tsuneko, as far as my research can tell, survives still, and is a prominent figure in the promotion of science in Japan.

They were able to discover the key to the mystery of the lagging strand by using a chased pulse technique, feeding E. coli irradiated nucleotides followed by non-irradiated nucleotides.

Sorry for using this space as a bully pulpit to vent my impotent rage and righteous indignation, but at least you know I still have plenty to say.

Matango! My Review

Matango! (Attack of the Mushroom People)
Color. 1963. 89 minutes, unrated. Directed by Ishiro Honda (famous for Godzilla and other kaiju films). You can watch the whole thing (in Japanese with English subtitles) here.
I recognized Akira Kubo from other Toho films, namely Destroy all Monsters, and Monster Zero. He was also in the Akira Kurosawa classic, Sanjuro. Other stars are also familiar Toho character actors. Also Yoshio Tsuchiya, also a star of several Godzilla films and Kurasawa classics. Kumi Mizuno, one of director Ishiro Honda's favorites.
The film begins like a dramatic version of Gilligan's Island. A ship with a passenger manifest including a professor, a singer, a plain jane, a mystery writer, and a millionaire (as well as a skipper and his flunky. Matango came out a year before Gilligan's Island.
The version I watched was dubbed and had subtitles. There were some interesting differences in the translation and the subtitles.
28 minutes in, we get to the first fungal reference. A derelict oceanographic ship covered in mold. Different colored mold in different parts of the ship. Radiation keeps the mold at bay. Thirty minutes in. We meet Matango, the giant mushroom. If only it were edible...
They are warned by the Captain's Log. DON'T eat the MUSHROOMS! They may contain nerve-damaging agents.
42 minutes in. More mushrooms. apparently growing on wood. "If you were starving, you'd eat them, wouldn't you?"
At 48 minutes, the first monster sighting. The damp! What happened? They seen the first mushroom man and then what happens?
1:09, The rain makes the mushrooms GROW.
1:13. The millionaire eats the mushrooms and starts tripping. The truth is revealed. Eat the mushrooms, become a mushroom. Oh. the laughing voices.
1:21. Apparently mushrooms are polite and knock before trying to ambush you. Is Matango a Russula? It breaks off pretty cleanly.
So how does it end? I don't want to spoil it.

As monster films go, this one wasn't particularly scary. I admit, the mushroom people don't have anything on Godzilla or any of the other kaiju. I do appreciate some of the touchs that I would expect from such a mycophilic culture as the Japanese. I did enjoy this movie more than I expected I would as a film. It tended to follow the Toho formula pretty well, dedicating almost half of the film to character development before the first tease of monster, with more and more monster footage leading up to the climax. It was easy to riff on it as I watched, a la MST3K. If you're a mycophile, it's definitely worth a watch, but over all I'd give it about 3 spores out of 5.

Matango!

Coming soon! My review of this film!

Statins

I visited a primary care physician for the first time in about four years. The last time I visited one was before coming to Alabama. My blood work came back showing that my previously OK cholesterol levels had gone ballistic. I attribute the jump to getting cozy with the southern diet. Too much BBQ, too many chicken fingers. So the bad news is that the good times of ignorantly eating and drinking to my heart's (dis)content are over. Better to know now than to find out in the emergency room, I suppose. So what does this have to do with fungi? It has to do with the prescription the doctor handed me.

As it turns out, the cholesterol-lowering wonder drugs of the 21st century, statins, were originally derived from fungi. Now mainly synthesized, these drugs work by inhibiting a key enzyme in cholesterol production, HMG-CoA reductase. Some of the first research on statins was performed by Dr. Akira Endo, a biochemist, who found cholesterol-inhibiting compounds in Penicillium citrinum. Penicillium spp., besides being very common mold agents, also gave us the first antibiotic discovery, penicillin.
Endo and Masao Kuroda hypothesized that Fungi could defend themselves from other organisms by inhibiting cholesterol production, and fortunately for millions of people, they were correct.
The ability to produce statins is apparently widespread throughout the true Fungi, even the oyster mushroom is naturally high in lovastatin (link to PDF). Antibiotics and statins, two of the most important medical discoveries of the past 100 years. Thanks, Fungi!


Update-1/19/10. I just found this article, which may help defuse one of the problems with statins, the grapefruit juice contraindication. The answer? more edible fungi, of course!

Cornell University to return fungal collection to China

In 1937, as the Japanese invaded China during World War II, a Chinese mycologist, educated in the United States, packed up some of the most prized specimens from a national botanic institute in Nanking. He loaded them on oxcarts and had them smuggled them out of the country to his alma mater in the 'States, Cornell University.

Now, these specimens are being repatriated including the rare Lentinus tigrinus, pictured above. (a local species of Lentinus is pictured in the masthead). A neat story you can read all about here. And here's a nice quote about the specimens from Cornell's Herbarium Director Kathie Hodge "To an average person, they look like something you would sweep off your kitchen floor. But under the microscope they're beautiful and exciting and incredibly diverse." How very true.

But of course, because the story is about fungi, it's filed under STRANGE (sigh).

March is Maitake Mushroom Month

From the "now you tell me department". Still a little bit of the Maitake Mushroom Month left, so go and get you some! I haven't had maitake in a long while. I've never seen them around Auburn, but I'm sure you can get them in Atlanta. I had some friends who used to grow maitake and other less well know cultivatable edibles out in California. Maitake is Japanese for the dancing mushroom, because finding this fungus fruiting led the finder to dance with joy. This mushroom can be found growing in the wilds of the Northeast and out to Idaho, according to Wikipedia.

Not much going on in the mushroom news...

The mushroom news has been rather dull of late. So I thought I'd amble over to see what's on YouTube if you search for mushrooms.
Here's what I thought was the pick of the bunch.

As usual, you can expect a lot of good fungal love from Japan. This is Shonen Knife with a song called "Brown Mushrooms", which isn't particularly descriptive. Lots of found mushrooms are often immediately shunted into Arora's "LBJ's" (Little Brown Jobs). But hey, at least I'm posting something.

Really, most of the mushroom news hasn't been too exciting of late.